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Proteomics

Proteomics Setup Small

High quality protein identification as well as accurate relative protein quantitation is done by nanoLC-MSMS. Reversed phase nano LC (Proxeon) using home made capillary columns results in peptide separations with a high resolution. MS spectra of the peptides are measured with an Orbitrap XL at approximately 5 ppm deviation or less. At the same time, MSMS spectra of the peptides are acquired in the LTQ. All measurements combined yield optimal protein identifications and relative quantitation.

N ESI Small

Peptide separations at a high chromatographic resolution: 

SjefChromatogram.jpg

High resolution Orbitrap MS spectra:

SjefMS_Spectrum.jpg

MSMS peptide fragmentation spectra:

SjefMSMS_Spectrum.jpg

SjefMaxQuant.jpg

Proteomics data clustering: 

SjefClustering.jpg